Reporter

Part:BBa_K337030

Designed by: Adlung L,Al Sabah J,Bayer P,Berrens R,Cristiano E,Flocke L,Kleinsorg S,Kolodziejczyk A,Macias Torre A,Mathur A,Mayilo D,Neumann S,Niopek D,Pisa R,Schad J,Schuhmacher L,Uhlig T,Wu X,Grimm D,Eils R   Group: iGEM10_Heidelberg   (2010-10-22)

Luc2_SV40 (microRNA binding site compatible)

Firefly luciferase Luc2 with XmaI/XhoI cutting sites in the 3'UTR enabling easy introduction of synthetic mircoRNA binding sites (BB-2, RFC12).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1944
    Illegal XhoI site found at 1686
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 70
    Illegal NgoMIV site found at 1414
    Illegal NgoMIV site found at 1435
    Illegal NgoMIV site found at 1707
    Illegal AgeI site found at 1138
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1320


[edit]
Categories
Parameters
None